Migration:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Microscopy:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Control:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Phospho-proteomics:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Western Blot:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Expressing:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Gene Expression:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Quantitative RT-PCR:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Activation Assay:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Translocation Assay:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Binding Assay:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
Blocking Assay:Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma.
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: Reactive Oxygen Species Drive Cell Migration and PD-L1 Expression via YB-1 Phosphorylation in Pleural Mesothelioma
Article Snippet: HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).HRP-conjugated rabbit secondary antibody (Cat. No. P0448) was obtained from Dako (Agilent Technologies, Santa Clara, CA, USA).. The RSK inhibitor BI-D1870 (Cat. No. sc-397022A) was procured from Santa Cruz Biotechnology (Dallas, TX, USA).. The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).The MEK inhibitor trametinib (Cat. No. HY-10999), the AKT inhibitor ipatasertib (Cat. No. HY-15186), and the YB-1 inhibitor SU056 (Cat. No. HY-150231) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).
Article Title: EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.
Article Snippet: The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.The pharmacological MEK inhibitors U0126 and PD98059, and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 were purchased from Merck Millipore.. Akt inhibitor MK-2206 was from ChemieTek, and RSK inhibitor BI-D1870 was from Santa Cruz Biotechnology.. We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal antibody against EphA2 (8B6) (Cell Signaling Technology); a mouse monoclonal antibody against HA (3F10) (Millipore); secondary antibodies conjugated to horseradish peroxidase (DAKO); Alexa Fluor 488-conjugated anti-GFP and Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific).We used the following antibodies in this study: a rabbit polyclonal antibody against EphA2 (C-20) (Santa Cruz Biotechnology); a mouse monoclonal antibody against -tubulin (Sigma); rabbit monoclonal antibodies against S897 phospho-EphA2 (D9A1) and T308 phospho-Akt (C31E5E), rabbit AC C EP TE D M AN U SC R IP T polyclonal antibodies against Akt, ERK, and T202/Y204 phospho-ERK, a mouse monoclonal..
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